Journal: Cancer cell

Article Title: MAST1 drives cisplatin resistance in human cancers by rewiring cRaf independent MEK activation

doi: 10.1016/j.ccell.2018.06.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-BIM (clone C34C5) antibody , Cell Signaling Technology , Cat#2933; RRID: AB_1030947.

Techniques: Recombinant, Negative Control, Viability Assay, Kinase Assay, Extraction, Ab Array, Activation Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, shRNA, Sequencing, Plasmid Preparation, Software

Journal: Cell metabolism

Article Title: Mitofusin 2 regulates axonal transport of calpastatin to prevent neuromuscular synaptic elimination in skeletal muscles

doi: 10.1016/j.cmet.2018.06.011

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Bim , Cell Signaling Technology , Cat# 2819S, RRID: AB_10692515.

Techniques: Histone Deacetylase Assay, Virus, Plasmid Preparation, Control, Recombinant, Ointment, Lysis, Western Blot, Transfection, Protease Inhibitor, Sample Prep, Transgenic Assay, Software

Journal: Cell metabolism

Article Title: Systematic dissection of the metabolic-apoptotic interface in AML reveals heme biosynthesis to be a regulator of drug sensitivity

doi: 10.1016/j.cmet.2019.01.011

Figure Lengend Snippet:

Article Snippet: Cat# ab14745, RRID:AB_2213640; Rabbit polyclonal anti-Bax (N-terminus) EMD Millipore Cat# 06–499, RRID:AB_310143 Rabbit monoclonal anti-Bcl-2 (clone D55G8) antibody Cell Signaling Technology Cat# 4223, RRID:AB_1903909 Rabbit monoclonal anti-Bcl-xL (clone 54H6) antibody Cell Signaling Technology Cat# 2764, RRID:AB_2228008 Rabbit monoclonal anti-Mcl-1 (clone S-19) antibody Santa Cruz Biotechnology Cat# sc-819, RRID:AB_2144105 Rabbit monoclonal anti-Bim (clone C34C5) antibody Cell Signaling Technology Cat# 2933, RRID:AB_1030947 Rabbit polyclonal anti-Bid antibody Cell Signaling Technology Cat# 2002, RRID:AB_10692485 Rabbit monoclonal anti-total Bad (clone D24A9) antibody Cell Signaling Technology Cat# 9239, RRID:AB_2062127 Rabbit monoclonal anti-phospho-Bad (S112) (clone 40A9) antibody Cell Signaling Technology Cat# 5284, RRID:AB_560884 Rabbit monoclonal anti-phospho-Bad (S136) (clone D25H8) antibody Cell Signaling Technology Cat# 4366, RRID:AB_10547878 Rabbit monoclonal anti-PUMA (clone D30C10) antibody Cell Signaling Technology Cat# 12450 Rabbit monoclonal anti-NOXA (clone D8L7U) antibody Cell Signaling Technology Cat# 14766 Bacterial and Virus Strains lentiCRISPR v2 Sanjana et al. Nat.

Techniques: Virus, Expressing, Plasmid Preparation, Recombinant, Cell Fractionation, Membrane, Cell Culture, Cell Viability Assay, Activity Assay, Isolation, shRNA, Metabolic Labelling, Software

Journal: Cell Proliferation

Article Title: The Neuroprotective Role of Ginsenoside Rg1 Against Cerebral Ischemia–Reperfusion Damage Through Inhibition of Mitophagy via Blocking Mitophagosome‐Lysosome Fusion

doi: 10.1111/cpr.70071

Figure Lengend Snippet: Inhibitory effect of Rg1 on mitophagy in OGD/R cells. (A) Representative transmission electron microscopy images showing mitochondrial ultrastructure in SK‐N‐AS cells after 12 h OGD and 24 h reperfusion, with or without 16 μM Rg1 treatment. Red arrows indicate double membrane vesicles with or without enclosed mitochondria (Scale bar: 5 μm). (B, C) Western blotting analysis of MFN1, MFN2, P62 and LC3 protein levels in SH‐SY5Y and SK‐N‐AS cells subjected to OGD followed by 12 or 24 h of reperfusion, with or without 16 μM Rg1 treatment. GAPDH served as the internal loading control. (D, E) The indicated proteins were evaluated in OGD‐exposed SH‐SY5Y and SK‐N‐AS cells after 24 h of reperfusion, with or without Rg1 treatment. Band quantification was performed using ImageJ software. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 versus DMSO group or indicated group.

Article Snippet: We used the following primary antibodies in our study: PINK1 (23274‐1‐AP, 1:1000), MFN1 (13798‐1‐AP, 1:1000), MFN2 (12186‐1‐AP, 1:1000), SQSTM1/P62 (18420‐1‐AP, 1:1000), and GAPDH (60004‐1‐Ig, 1:10000) from Proteintech (Hubei, China); BNIP3L (A6283, 1:1000) and FUNDC1 (A16318, 1:1000) from ABclonal (Hubei, China); Parkin (2132, 1:1000) from Cell Signalling Technology (MA, USA); and LC3 (L7543, 1:1000) from Sigma‐Aldrich (MO, USA).

Techniques: Transmission Assay, Electron Microscopy, Membrane, Western Blot, Control, Software

Journal: Cell Proliferation

Article Title: The Neuroprotective Role of Ginsenoside Rg1 Against Cerebral Ischemia–Reperfusion Damage Through Inhibition of Mitophagy via Blocking Mitophagosome‐Lysosome Fusion

doi: 10.1111/cpr.70071

Figure Lengend Snippet: Neuroprotective effect of ginsenoside Rg1 are mediated through the regulation of mitophagy. (A) SK‐N‐AS cells subjected to OGD/R were treated with DMSO, 16 μM Rg1, 20 μM Mdivi‐1 (a mitophagy inhibitor), 10 μM CCCP (a mitophagy inducer), or a combination of CCCP and Rg1. The immunofluorescence assay showed co‐localisation of mitochondria (MitoTracker, green) and lysosomes (LysoTracker, red), with DAPI (blue) highlighting the cell nuclei (Scale bar: 10 μm). (B, C) SH‐SY5Y and SK‐N‐AS cells subjected to OGD followed by 24 h of reperfusion were treated with the indicated substances. The expression levels of MFN1 and MFN2 were analysed by Western blotting. Cell viability inhibition under various treatment conditions was evaluated using the CCK‐8 assay (D, E), and the ratio of cell death was determined by the PI exclusion assay (F, G). #### p < 0.0001 versus Ctrl group. *p < 0.05; **p < 0.01; *** p < 0.001; **** p < 0.0001 versus DMSO group or indicated group. “NS” indicates no significant difference.

Article Snippet: We used the following primary antibodies in our study: PINK1 (23274‐1‐AP, 1:1000), MFN1 (13798‐1‐AP, 1:1000), MFN2 (12186‐1‐AP, 1:1000), SQSTM1/P62 (18420‐1‐AP, 1:1000), and GAPDH (60004‐1‐Ig, 1:10000) from Proteintech (Hubei, China); BNIP3L (A6283, 1:1000) and FUNDC1 (A16318, 1:1000) from ABclonal (Hubei, China); Parkin (2132, 1:1000) from Cell Signalling Technology (MA, USA); and LC3 (L7543, 1:1000) from Sigma‐Aldrich (MO, USA).

Techniques: Immunofluorescence, Expressing, Western Blot, Inhibition, CCK-8 Assay, Exclusion Assay

Journal: Cell Proliferation

Article Title: The Neuroprotective Role of Ginsenoside Rg1 Against Cerebral Ischemia–Reperfusion Damage Through Inhibition of Mitophagy via Blocking Mitophagosome‐Lysosome Fusion

doi: 10.1111/cpr.70071

Figure Lengend Snippet: Inhibitory effect of Rg1 on mitophagy in MCAO/R mice. (A) IHC analysis of MFN1, MFN2 and P62 expression in brain tissues from MCAO/R and Rg1‐treated groups. Representative images and quantification of integrated optical density show protein expression in the entire ipsilateral infarct area. Scale bars: 1000 μm and 20 μm. (B) Representative images of double immunofluorescence staining of NeuN (neuronal marker, red) and MFN1 (mitophagy marker, green), with co‐localisation quantitative analysis. DAPI (blue) was used to indicate cell nuclei. Scale bar: 20 μm. All results are presented as mean ± SD and were replicated at least three times. Statistical significance was indicated as follows: ** p < 0.01; **** p < 0.0001 compared to the MCAO/R group.

Article Snippet: We used the following primary antibodies in our study: PINK1 (23274‐1‐AP, 1:1000), MFN1 (13798‐1‐AP, 1:1000), MFN2 (12186‐1‐AP, 1:1000), SQSTM1/P62 (18420‐1‐AP, 1:1000), and GAPDH (60004‐1‐Ig, 1:10000) from Proteintech (Hubei, China); BNIP3L (A6283, 1:1000) and FUNDC1 (A16318, 1:1000) from ABclonal (Hubei, China); Parkin (2132, 1:1000) from Cell Signalling Technology (MA, USA); and LC3 (L7543, 1:1000) from Sigma‐Aldrich (MO, USA).

Techniques: Expressing, Double Immunofluorescence Staining, Marker

Journal: Cell reports

Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases

doi: 10.1016/j.celrep.2019.05.061

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-Bim (C34C5) Al488 , Cell Signaling , Cat#94805S; RRID not available.

Techniques: Virus, Plasmid Preparation, Recombinant, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Software, Extraction, Gene Expression